A previous study demonstrated that animal erythrocytes such as horse, dog, rabbit and
rat were agglutinated by the saline extracts of Arisaema amurense Maximowicz (AAM)
while human erythrocytes were not by one of authors (Moon). In the present
experiments, normal cells such as human erythrocytes, leukocytes, lung, liver, spleen,
kidney, heart, stomach, adrenal gland and urinary bladder cells were suspended in 0.1%
EDTA solution and tumor cells such as acute & chronic myelocytic leukemia, acute
lymphocytic leukemia, lung cancer, urinary bladder cancer, mice leukemia cells and
leukocytes of multiple myeloma patients were suspended in 0.1% EDPA solution and
performed agglutination test with AAM phytagglutinin which were extracted by various
solvents such as saline, ammonium sulfate(AS ; 45%, 75% and 85% solution) and
perchloric acid (PA : 0.45M, 0.75M and 1.5M solution).
The effects of the saliva and sugars on agglutination inhibition were studied as well in
the present paper with the following results
Normal cells were not agglutinated by AAM saline extracts while some of non
leukemia patient's leukocytes were agglutinated by AS an4 PA extracts.
Tumor cells used in this experiment were highly susceptible to agglutination by AAM
extracts in order PA, AS and saline extracts.
AAM saline and AS extracts induced agglutination of tumor cells were inhibited by
D-galactose and PA extracts were by L-fucose.
All kinds of AAM extracts such as saline AS and PA induced agglutinations of tumor
cells were inhibited by some human saliva and some were not and it was not related
with secrete form and blood groups of saliva.
These results indicate that AAM agglutinability by various extraction emphasizes the
importance of examining a wide variety of cells in attempting to understand the
interactions of PHA with an cell surface and AAM extracts are more useful than con-A
and wheat germ agglutinin to study for cellular surface recept sites of tumor cells.
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